Research Associate - Specialism in Wetlab with potential Bioinformatics experience

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Overview

Do you have a passion for immunology, RNA and gene-regulation? We are seeking an outstanding Postdoctoral Researcher to join an ambitious interdisciplinary programme at the University of Sheffield investigating how RNA regulation controls innate immune responses during inflammation.

You will have a PhD in an appropriate subject and a keen interest in RNA biology, innate immunity or in vivo modelling. This position is suited to someone with the ability and motivation to design and perform complex experimental procedures, including high-throughput RNA focused experiments, as well as analysing the results. The project will generate extensive multi-omics datasets, therefore experience in, or aptitude for, bioinformatic analysis would be advantageous.

You will form part of a team that will combine primary human neutrophil culture and zebrafish models with the latest molecular biology, genomics, bioinformatics and in vivo imaging to investigate how ELAVL1, a major RNA-binding protein, impacts neutrophil biology. We will uncover the fundamental principles of immune regulation by post-transcriptional regulation, with relevance to inflammatory and infectious diseases.

You will be able to demonstrate a collaborative mindset, be looking to acquire and use new skills and enjoy working in an interdisciplinary way. You will look forward to being part of a community of researchers working towards common goals.

This is an exceptional opportunity for ambitious researchers to work at the forefront of RNA biology and immunology, access world-class facilities, contribute to high-impact discoveries, and develop an independent research profile within a collaborative and supportive research environment.

Main Duties And Responsibilities

We are seeking a highly motivated Postdoctoral Research Associates to join a multidisciplinary research programme investigating the regulation and function of ELAVL1 in neutrophil biology and inflammation. The project combines molecular biology, genomics, bioinformatics, and in vivo disease models to uncover mechanisms controlling RNA processing and immune cell function. The successful candidate will work in a team where complementary expertise will be available to cover all required skills and will contribute to complementary work packages spanning mechanistic studies in human cells and functional investigations in zebrafish models.

Main Duties And Responsibilities

• Design and conduct independent research into the molecular mechanisms regulating ELAVL1 function in innate immune cells.
• Culture and experimentally manipulate primary human neutrophils and related cellular models.
• Perform and analyse high-throughput -omics experiments, including RNA sequencing (RNA-seq), eCLIP, SLAM-seq, RIP-seq, and associated transcriptomic datasets in vitro and in vivo.
• Investigate post-transcriptional regulatory mechanisms, including intron retention, mRNA stability, RNA editing, methylation, and protein phosphorylation.
• Develop and apply molecular biology approaches such as CRISPR-Cas9 genome editing, RNA affinity pull-down assays, mutant analysis, and gene perturbation strategies.
• Undertake in vivo studies using zebrafish models, including inflammation and infection assays, live imaging, and functional analysis of genetic mutants.
• For suitable applicants, there will be an opportunity to generate, integrate, and interpret large-scale multi-omics datasets, identifying biologically relevant pathways and regulatory networks.
• Use a range of computational tools for data processing, statistical analysis, visualisation, and cross-referencing of multiple datasets.
• Contribute to experimental design, data interpretation, and dissemination of research findings through publications, presentations, and reports.
• Maintain accurate experimental records and comply with laboratory, ethical, and health and safety procedures.
• Collaborate effectively with researchers across molecular biology, bioinformatics, and imaging disciplines, and support the training of students and junior researchers where appropriate.
• Carry out other duties, commensurate with the grade and remit of the post