The Role

This position will focus on the development of improved methodology in high pressure freezing for in-situ structural Cell Biology, where molecules are now placed in its physiological context in cells, organoids, and tissues. This is achieved through imaging of cryo-preserved samples while maintaining samples in their native state. It is important to emphasise that the critical first step in this workflow is always Cryo preservation, a technique that requires critical development to fully support the demands of the cryo-EM field. This Chan Zuckerberg Initiative (CZI) funded project allows us to address some of the issues and develop new tools and workflows to improve the success rate for HPF. The project will focus on cells grown directly on EM grids adapting the “waffle” method but there is scope to look at HPF technology in general.

This vacancy is for a 1 FTE position. This would be subject to our Flexible working policy.

What will you be doing?

You will be responsible for performing electron microscopy experiments, from preparing samples to analysis, both TEM and SEM based.

Standard Responsibilities:

• Responsible for the effective execution of EM experiments performed as part of the research carried out in the Verkade research group.
• Develop and maintain familiarity with the project aims and an awareness of the research area.
• Collate and report on research or project outputs as required compiling or writing initial versions of communications and reports.
• Plan and organise own work activities as well as support those of others to ensure that deadlines are met.
• Work in partnership with key (internal and external) stakeholders to co-ordinate resource allocation, work scheduling and provide advice and support to all staff and students linked to the project and Verkade research group.