Barrington James
Molecular Cloning & Protein Engineering Scientist
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You will be responsible for engineering and building therapeutic constructs. This is a hands-on, high throughput role where you will design, build and iterate on novel CRISPR-Based constructs that enable a programmable, target-selective approach across multiple oncology indications. Your work will directly shape the therapeutic candidates that enter preclinical development.
Responsibilities
- Design, engineer, and optimize Cas-based therapeutic constructs, including conditional and programmable protein architectures.
- Engineer proteins for enhanced specificity, activity, and control using rational design, structure-guided engineering, and directed evolution.
- Expand the modularity of the platform by developing constructs targeting additional disease-relevant genes.
- Design, clone, and validate guide RNA libraries for specificity and off-target profiling assays (e.g., ONE-seq, CHANGE-seq, or related technologies).
- Design, execute, and analyze rapid design-build-test cycles to improve construct performance.
- Collaborate across molecular biology, protein engineering, computational biology, and translational research teams to advance therapeutic programs.
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Required Qualifications
- PhD in Synthetic Biology
- At least 5 years of hands-on experience in molecular cloning using techniques such as Gibson Assembly, Golden Gate Assembly, and Gateway cloning.
- At least 5 years experience of Protein engineering (engineered the function, not just been apart)
- Extensive experience designing and engineering CRISPR/Cas constructs that enable a programmable, target-selective approach across multiple oncology indications, including development of novel protein functions through directed evolution or rational engineering.
- Strong expertise in mammalian and bacterial expression vector design, construct optimization, and stable cell line generation.
- Demonstrated ability to independently execute rapid iterative construct design and optimization.
- Experience developing and utilizing fluorescent and/or luminescent reporter assay systems.
- Strong structural biology and wet-lab expertise, with experience applying structural insights to protein engineering challenges.
- Demonstrated ownership of engineering protein function, rather than solely supporting execution of established workflows.
- Computational experience supporting protein or CRISPR engineering, including sequence analysis, structural modeling, guide RNA design, scripting, or bioinformatics.
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Preferred Qualifications
- Experience with inducible or conditional protein systems.
- Familiarity with base editing, prime editing, or other next-generation genome editing technologies.
- Experience with NGS library preparation and sequencing-based screening workflows.
- Background in structural biology, protein biochemistry, or computational protein engineering.
- Experience working in fast-paced biotechnology or therapeutic discovery environments.
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